Computes the lower-Tm-minus5°C gradient starting point in NEB general PCR troubleshooting guidance. Input Tm values must already reflect applicable salt/primer conditions. Does not predict Tm from sequences or guarantee optimum temperature/success;polymerase-specific instructions take precedence.
Computes the lower-Tm-minus5°C gradient starting point in NEB general PCR troubleshooting guidance. Input Tm values must already reflect applicable salt/primer conditions. Does not predict Tm from sequences or guarantee optimum temperature/success;polymerase-specific instructions take precedence.
Enter your values and run the tool to see your result.
Check the units, enter your values, and run the tool. Results follow the assumptions and method below.
min(forward,reverse)-5abs(forward-reverse)